| 1 |
Laboratory equipment, preparation of solutions and buffers |
|
| 2 |
Growing Bacteria and fungi |
|
| 3 |
Genomic DNA extraction from bacteria |
|
| 4 |
Genomic DNA extraction from fungi |
|
| 5 |
ITS and 16S PCR and electrophoresis |
|
| 6 |
PCR-RFLP |
|
| 7 |
Plasmid extraction |
|
| 8 |
Molecular cloning |
|
| 9 |
Competent cell preparation and Transformation |
|
| 10 |
Colony-PCR and sequencing |
|
| 11 |
Bacterial RNA extraction |
|
| 12 |
Recombinant protein expression in bacteria |
|